Difference between revisions of "BME103:T130 Group 7 l2"

From OpenWetWare
Jump to: navigation, search
(Thermal Cycler Engineering)
m (Thermal Cycler Engineering)
Line 32: Line 32:
  
 
[[Image:Roof Assembly small.png]]
 
[[Image:Roof Assembly small.png]]
 +
[[Image:Plate with Button.png]]
 
[[Image:Plastic Body Final.png]]
 
[[Image:Plastic Body Final.png]]
[[Image:Plate with Button.png]]
 
 
[[Image:Power Cord Final.png]]
 
[[Image:Power Cord Final.png]]
 
[[Image:32 wells final.png]]
 
[[Image:32 wells final.png]]

Revision as of 12:50, 27 November 2012

Owwnotebook icon.png BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
BME494 Asu logo.png

OUR TEAM

Name: Emily Thompson
Research & Development Scientist
Name: Vivian Benjes
Experimental Protocol Planner/ Data Analyst
Name: Frances Marrett
Experimental Protocol Planner
Name: Chris Glass
Open PCR Machine Engineer
Name: Ryan Frantz
Open PCR Machine Engineer
[1]
Name: Cenric Nigbur
Ghost/ TBD

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design

Roof Assembly small.png Plate with Button.png Plastic Body Final.png Power Cord Final.png 32 wells final.png

Key Features


Instructions





Protocols

Materials


Supplied in the Kit

Thermal Cycler for PCR reaction
Qiagen Fast Cycling PCR kit (50)
BioRad Real Time PCR Applications Guide


Supplied by User

Sterile gloves
Access to 120V electrical outlet
10 uL pipette
Eppendorf tube block

PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers
Alzheimer's disease, a form of dimentia in which brain function is lost gradually over time, is associated with the SNP rs121918396. The sequence associated with Alzheimer's is TAG, while a normal sequence is TGG.



Primer Design



Illustration