BME103:T130 Group 13 l2: Difference between revisions
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To run the PCR test first set up the PCR machine by plugging it into an electrical outlet and downloading the smartphone application. Program the application to fit the needs of your test, set the temperature, and number of cycles needed for your test. The average test will take about two hours to run. The row in the PCR machine has two sample spots, these spots are designated for a positive and negative control. For the positive control sample, add the DNA cancer template sample with the reagent and for the negative, add the negative sample with the reagent. There are six more rows with three sample spots available for six different patient samples. It is useful to have three samples for each patient to get a more reliable result. The final volume of the reaction should be 2 mL. Let the cycles run to completion for results. | To run the PCR test first set up the PCR machine by plugging it into an electrical outlet and downloading the smartphone application. Program the application to fit the needs of your test, set the temperature, and number of cycles needed for your test. The average test will take about two hours to run. The row in the PCR machine has two sample spots, these spots are designated for a positive and negative control. For the positive control sample, add the DNA cancer template sample with the reagent and for the negative, add the negative sample with the reagent. There are six more rows with three sample spots available for six different patient samples. It is useful to have three samples for each patient to get a more reliable result. The final volume of the reaction should be 2 mL. Let the cycles run to completion for results. | ||
Adjusting smartphone settings: | '''Adjusting smartphone settings:''' | ||
<br>1) | <br>1)First inactivate the flash. | ||
<br>2) | <br>2)Set ISO to 800. | ||
<br>3) | <br>3)Set white balance to auto. | ||
<br>4) | <br>4)Set exposure to highest setting. | ||
<br>5) | <br>5)Set saturation to the highest setting. | ||
<br>6) | <br>6)Set contrast to lowest setting. | ||
'''DNA Measurement Protocol'''<br> | '''DNA Measurement Protocol'''<br> |
Revision as of 00:56, 21 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol Adjusting smartphone settings:
DNA Measurement Protocol Research and DevelopmentBackground on Disease Markers
Illustration
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