BME103:T130 Group 12: Difference between revisions
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A '''polymerase chain reaction''' (PCR) is based on the enzyme DNA Polymerase's ability to synthesize complementary DNA strands. Through a series of steps involving polymerase breaking apart a DNA strand and then synthesizing a specified complementary piece, a PCR machine is able to isolate and amplify a desired strand of DNA. <br> | A '''polymerase chain reaction''' (PCR) is based on the enzyme DNA Polymerase's ability to synthesize complementary DNA strands. Through a series of steps involving polymerase breaking apart a DNA strand and then synthesizing a specified complementary piece, a PCR machine is able to isolate and amplify a desired strand of DNA. <br> | ||
'''Steps to Amplify a Patient's DNA Sample''' <br> | '''Steps to Amplify a Patient's DNA Sample''' <br> | ||
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'''4.''' And step 4 is not in Russian | '''4.''' And step 4 is not in Russian | ||
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'''Components of PCR Master Mix''' | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | | align="center" style="background:#f0f0f0;"|'''Reagent''' | ||
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| GoTaq master mix||50.0μL | | GoTaq master mix||50.0μL | ||
|- | |- | ||
| | | dH<sub>2</sub>O||47.8μL | ||
|- | |- | ||
| Total Volume||100μL | | '''Total Volume'''||100μL | ||
|} | |} | ||
Revision as of 14:54, 1 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction A polymerase chain reaction (PCR) is based on the enzyme DNA Polymerase's ability to synthesize complementary DNA strands. Through a series of steps involving polymerase breaking apart a DNA strand and then synthesizing a specified complementary piece, a PCR machine is able to isolate and amplify a desired strand of DNA.
Steps to Amplify a Patient's DNA Sample 1. This is step 1 2. This is where step 2 will go 3. Здравствуйте! 4. And step 4 is not in Russian |
Reagent | Volume |
Template DNA (20 ng) | 0.2μL |
10μM forward primer | 1.0μL |
10μM reverse primer | 1.0μL |
GoTaq master mix | 50.0μL |
dH2O | 47.8μL |
Total Volume | 100μL |
Flourimeter Measurements
(Add your work from Week 3, Part 2 here)
Research and Development
Specific Cancer Marker Detection - The Underlying Technology
(Add a write-up of the information discussed in Week 3's class)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
(Your group will add the results of your Fluorimeter measurements from Week 4 here)