Difference between revisions of "BME103:Logistics"

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** 3 Lab sections, ~85 students per section
 
** 3 Lab sections, ~85 students per section
 
*** 10 - 17 groups, of ~5 students per group
 
*** 10 - 17 groups, of ~5 students per group
* 3 hours per lab
+
* 3 hours per class meeting
 
<br>
 
<br>
  
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'''MATERIALS & METHODS - DNA AMPLIFICATION'''
 
'''MATERIALS & METHODS - DNA AMPLIFICATION'''
 
* Reactions (100 μL total volume) contained:
 
* Reactions (100 μL total volume) contained:
** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
+
** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127]) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
 
** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
 
** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
 
** 2x GoTaq colorless PCR Master Mix - Promega M7133
 
** 2x GoTaq colorless PCR Master Mix - Promega M7133

Latest revision as of 16:12, 2 January 2013

Owwnotebook icon.png BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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COURSE SPECS

  • ~230 Freshmen
    • 3 Lab sections, ~85 students per section
      • 10 - 17 groups, of ~5 students per group
  • 3 hours per class meeting


TEMPLATE PAGES


MATERIALS & METHODS - DNA AMPLIFICATION

  • Reactions (100 μL total volume) contained:
    • Template DNA - Kozak (BBa_BBa_J176012) inserted in plasmid vector V0120 (BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
    • Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
    • 2x GoTaq colorless PCR Master Mix - Promega M7133
  • A few of the samples were checked via standard ethidium bromide-stained agarose gel electrophoresis outside of class (see [1]). All other samples were measured via SYBRgreen staining on an LED fluorimeter.
  • All reactions were run on an Open PCR machine.


MATERIALS & METHODS - DNA IMAGING

  • Details to be added soon


OBTAINING MATERIALS

  • We have written several worksheets and hand outs for this course.
  • Please contact one of the instructors if you are interested in obtaining copies.