Difference between revisions of "BISC220/S13: Media Recipes"

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(New page: {{Template:BISC220/S13}} <div style="padding: 10px; width: 720px; border: 5px solid #E05D1B;"> == Apoptosis Series Media Recipes == {| border="1" |+ ! Solution !! Composition |- ! PBS ...)
 
(Apoptosis Series Media Recipes)
 
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! anti-PARP-1 antibody (mouse)
 
! anti-PARP-1 antibody (mouse)
 
| diluted 1:1000  
 
| diluted 1:1000  
 +
|-
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! Immun-Star™ WesternC™ Chemiluminescence Kit
 +
| www.bio-rad.com <br>  Catalog #170-5070
 +
|-
 +
! Precision Plus Protein™ WesternC™ Standards (Ladder)
 +
| www.bio-rad.com <br> Catalog #161-0385
 
|-
 
|-
 
|}
 
|}

Latest revision as of 11:16, 8 April 2013

Wellesley College     BISC 220     Cellular Physiology

BISC220 S10 template image.png

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Enzymes        Secretory Pathway        Apoptosis        OWW Basics

Apoptosis Series Media Recipes

Solution Composition
PBS (Phosphate Buffered Saline) 1X 2.68 mM KCl
1.46 mM KH2PO4
136.9 NaCl
8.1 mM Na2HPO4
PBST (PBS with Tween 20) 1X PBS with 0.25% Tween 20
Trypsin Stock = 0.25%
Cell Culture Media 1 Packet DMEM Powder
0.15% NaHCO3
10% Newborn Calf Serum
Trypan Blue 0.1% Trypan Blue in water
Etoposide 100 mM in DMSO (stock)
500 μM (effective)
DNA extraction buffer 50 mM Tris pH 8.0
20 mM Na2EDTA
10 mM NaCl
1% SDS
20 ug/ml RNase A
100 ug/ml Proteinase K
PMSF/glucose buffer (protein buffer 1) 50 mM glucose
25 mM Tris pH 8.0
10 mM Na2EDTA
and 1 mM PMSF
urea/SDS buffer (protein buffer 2) 50 mM Tris pH 6.8
6M urea
6% B-ME
3% SDS
0.003% bromophenol blue
anti-PARP-1 antibody (mouse) diluted 1:1000
Immun-Star™ WesternC™ Chemiluminescence Kit www.bio-rad.com
Catalog #170-5070
Precision Plus Protein™ WesternC™ Standards (Ladder) www.bio-rad.com
Catalog #161-0385