|Point Value |
||Complete the 4 question pre-lab quiz prior to coming to lab each week. The pre-lab quiz will be available 3 days before lab and closes at the start of lab each week. No late quizzes are allowed and there are no make-ups.
||2/week (20 total)
||Complete entry assessment (2.5pts); Submit preliminary cross results Day 3 (1 pt);
Summarize experimental question, design; Diagram crosses using template provided; Explain data interpretation (9 pts). This assignment is described at: BISC219/F13: Assignment_1_Lab1
Results Section BISC219/F13: Assignment Help- Data Analysis 1
Autosomal vs. Sex linked inheritance
||Read Background information on your Series 2 Forward Genetics Project at BISC219/F13:_Gene_Mapping_Info. Submit statement of Series 2 experimental question & goals. Outline (or create flow diagram) our experimental process including : Mutant Phenotype description compared to WT; Linkage Analysis; Mapping the Mutation to a chromosome and to a particular chromosomal location; Complementation Analysis; Defining the gene sequence and protein change through DNA sequence analysis; & Bioinformatics to find homologs and to determine if the mutation is new or previously characterized. Write a narrative summary of the investigation.
Diagram the expected results (genotype and phenotype)for the crosses and self-fertilization progeny in the Linkage Testing work. Use only part (through Linkage 2) of the template provided. (The Mapping part of the template will be used next week). Include a narrative description of this experimental design that you have drawn out on the template and summarize what you will be looking for in the results that will allow determination of the linkage group to which your mutated gene of interest belongs. Find a rubric and more information about this assignment at: BISC219/F13: Assignment_Series2_Outline_Summary.
||Explain complementation analysis in general and then explain specifically how you will use it in this investigation. Draw out the crosses that you set up using the templates provided. Assignment information and rubric found at: BISC219/F13: Assignment_Series2_Complementation
||Diagram the expected results (genotype and phenotype)for the crosses and self-fertilization progeny in the Mapping work that you will do over the next several weeks by completing the templates provided. Make sure you highlight the progeny that will be used to continue each step of the process. Assignment information and rubric found at: BISC219/F13: Assignment_Series2_Mapping Crosses.
||Analyze your mutant gene's DNA sequence using the instructions that you can download from Lab 6. Construct a Results and Discussion section, WITH A FIGURE, for this analysis. Assignment information and rubric found at: BISC219/F13: Assignment_Series2_DNA Sequencing
||Scientific Research Report on Series2 work Classical (Forward) Genetics. Assignment information found at BISC219/F13: Assignment_ Series2_Classical Genetics Paper
||Convert all Series3 Protocols, performed so far, as Materials & Methods (7.5) and write a draft introduction section ((7.5). Include properly cited references and a properly formatted Literature Cited page) section in this intro draft. This Assignment is described at BISC219/F13: Assignment_Series3_ Materials and Methods_Introduction. Also refer to BISC219/F13:Resources Guide to Scientific Writing. Bring hard copies of the journal articles you referenced in your draft Introduction to use during a science writing workshop and concept discussion in Lab11 and be prepared to lead the class discussion or summarize the major points of the articles.
||Construct figures/tables with properly formatted legends to illustrate the main findings of the RNAi part of your Series3:Reverse Genetics project (5pts). These figures are a draft of those that will be part of the results section of the research report you will write for Series3.
|Last Day of Classes
|Full scientific research report due last day of classes for all students. Assignment information found at BISC219/F13: Assignment_Series3_Reverse Genetics Paper using RNAi
||Exit Assessment participation. See Sakai Announcement for link and for instructions on how to receive your incentive points (2.5).