BISC219/F12:RNA interference
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These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the Silencing Genomes project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.
Background Information on Project 3: Investigating Gene Regulation Using RNAi
Media Recipes
Lab 7:
Lab 8:
Lab 9:
Lab 10:
Lab 11:
Lab 12:
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 7 | 10/23 - 10/29 | Picking a colony of bacteria containing the RNAi plasmid | The night before next lab: set up an overnight culture of your colony of interest |
| 8 | 10/30 - 11/5 | PCR to confirm presence of gene of interest, miniprep of plasmid and transformation into feeding bacteria strain | The day after lab: check your transformed bacteria for colony growth The night before next lab: set up an overnight culture of your colony of interest |
| 9 | 11/7 - 11/13 | Induction of dsRNA production, seeding of RNAi plates and addition of worms | |
| 10 | 11/14 - 11/20 | Chemotaxis Assay | |
| 11 | 11/26 - 11/30 | Writing Workshop | |
| 12 | 12/3 - 12/7 | Writing Conferences |
