Difference between revisions of "BISC219/F12:Gene Mapping"

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(New page: {{Template:BISC219/F12}} <div style="padding: 10px; width: 720px; border: 5px solid #7e354d;"> Background: Classical Forward Genetics and Gene Mapping...)
 
(Schedule of Experiments for Series 2)
 
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! 2
 
! 2
|  
+
| 9/11 - 9/17
 
| Picking mutant worms for genetic mapping
 
| Picking mutant worms for genetic mapping
 
| Confirm mutant phenotype and set up first cross
 
| Confirm mutant phenotype and set up first cross
 
|-
 
|-
 
! 3
 
! 3
|
+
| 9/18 - 9/24
 
| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites
 
| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites
 
| Pick hermaphrodites for selfing
 
| Pick hermaphrodites for selfing
 
|-
 
|-
 
! 4
 
! 4
|  
+
| 9/25 - 10/1
 
| Examine phenotypes to determine linkage<br>
 
| Examine phenotypes to determine linkage<br>
 
'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males
 
'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males
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! 5
 
! 5
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+
| 10/2 - 10/5 (10/15 Mon. Lab)
 
| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''
 
| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''
 
| Nothing! Enjoy Fall Break!
 
| Nothing! Enjoy Fall Break!
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|-
 
|-
 
! 6
 
! 6
|  
+
| 10/16 - 10/22
 
| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)  
 
| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)  
 
| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)  
 
| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)  
 
|-
 
|-
 
! 7
 
! 7
|  
+
| 10/23 - 10/29
 
| ''' Complete Mapping'''<br>
 
| ''' Complete Mapping'''<br>
 
SCORE! Find recombinantion frequency
 
SCORE! Find recombinantion frequency
 
| Determine map distance between your Dpy and Unc marker<br>
 
| Determine map distance between your Dpy and Unc marker<br>
 
|-
 
|-

Latest revision as of 06:39, 17 August 2012

Background: Classical Forward Genetics and Gene Mapping
Lab 2: Examining mutant worms for mapping
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2

Lab # Dates Activity Outside Activity
2 9/11 - 9/17 Picking mutant worms for genetic mapping Confirm mutant phenotype and set up first cross
3 9/18 - 9/24 Linkage crosses - heterozygous males with Unc hermaphrodites Pick hermaphrodites for selfing
4 9/25 - 10/1 Examine phenotypes to determine linkage

Mapping: Pick Unc non-Dpy to separate plates;
Complementation: cross unknown Dpy worms to wild type males

Mapping: Pick putative double mutants
Complementation: cross heterozygous males with known Dpy mutants
5 10/2 - 10/5 (10/15 Mon. Lab) Mapping: Maintain double mutants (d u/d u): pick a few to individual plates
Complementation: examine crosses for Dpy MALES
Nothing! Enjoy Fall Break!
6 10/16 - 10/22 DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;
Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species;
Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
7 10/23 - 10/29 Complete Mapping

SCORE! Find recombinantion frequency

Determine map distance between your Dpy and Unc marker