BISC219/F12:Gene Mapping: Difference between revisions
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(New page: {{Template:BISC219/F12}} <div style="padding: 10px; width: 720px; border: 5px solid #7e354d;"> Background: Classical Forward Genetics and Gene Mapping...) |
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|- | |- | ||
! 2 | ! 2 | ||
| | | 9/11 - 9/17 | ||
| Picking mutant worms for genetic mapping | | Picking mutant worms for genetic mapping | ||
| Confirm mutant phenotype and set up first cross | | Confirm mutant phenotype and set up first cross | ||
|- | |- | ||
! 3 | ! 3 | ||
| | | 9/18 - 9/24 | ||
| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites | | '''Linkage''' crosses - heterozygous males with Unc hermaphrodites | ||
| Pick hermaphrodites for selfing | | Pick hermaphrodites for selfing | ||
|- | |- | ||
! 4 | ! 4 | ||
| | | 9/25 - 10/1 | ||
| Examine phenotypes to determine linkage<br> | | Examine phenotypes to determine linkage<br> | ||
'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males | '''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males | ||
| Line 33: | Line 33: | ||
|- | |- | ||
! 5 | ! 5 | ||
| | | 10/2 - 10/5 (10/15 Mon. Lab) | ||
| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES''' | | '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES''' | ||
| Nothing! Enjoy Fall Break! | | Nothing! Enjoy Fall Break! | ||
| Line 39: | Line 39: | ||
|- | |- | ||
! 6 | ! 6 | ||
| | | 10/16 - 10/22 | ||
| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du) | | ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du) | ||
| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u) | | '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u) | ||
|- | |- | ||
! 7 | ! 7 | ||
| | | 10/23 - 10/29 | ||
| ''' Complete Mapping'''<br> | | ''' Complete Mapping'''<br> | ||
SCORE! Find recombinantion frequency | SCORE! Find recombinantion frequency | ||
| Determine map distance between your Dpy and Unc marker<br> | | Determine map distance between your Dpy and Unc marker<br> | ||
|- | |- | ||
Latest revision as of 07:39, 17 August 2012
Background: Classical Forward Genetics and Gene Mapping
Lab 2: Examining mutant worms for mapping
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!
Schedule of Experiments for Series 2
| Lab # | Dates | Activity | Outside Activity |
|---|---|---|---|
| 2 | 9/11 - 9/17 | Picking mutant worms for genetic mapping | Confirm mutant phenotype and set up first cross |
| 3 | 9/18 - 9/24 | Linkage crosses - heterozygous males with Unc hermaphrodites | Pick hermaphrodites for selfing |
| 4 | 9/25 - 10/1 | Examine phenotypes to determine linkage Mapping: Pick Unc non-Dpy to separate plates; |
Mapping: Pick putative double mutants Complementation: cross heterozygous males with known Dpy mutants |
| 5 | 10/2 - 10/5 (10/15 Mon. Lab) | Mapping: Maintain double mutants (d u/d u): pick a few to individual plates Complementation: examine crosses for Dpy MALES |
Nothing! Enjoy Fall Break! |
| 6 | 10/16 - 10/22 | DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du) |
Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species; Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u) |
| 7 | 10/23 - 10/29 | Complete Mapping SCORE! Find recombinantion frequency |
Determine map distance between your Dpy and Unc marker |
