|Practice metric conversions;
Making Solutions; Microscopy;
Examination of Tetrahymena pyriformis
|Homework: Familiarize yourself with the wiki, particularly the information in the BISC110/S12:Resources section of the Lab wiki; |
Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_Problems110.doc;
Read about the process of endocytosis in your textbook;
familiarize yourself with phagocytosis in Tetrahymena
from the following reference articles:
*Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate Tetrahymena vorax,
the non-selective phagocytosis seen in microstomes
changes to a highly selective process in macrostomes.
(2002), Journal of Experimental Biology 205, 2089-2097;
*McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin,
and centrin to the oral apparatus and buccal cavity of the macrostomal form of Tetrahymena vorax., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
|Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis;
Accuracy vs. Precision; Statistical Parameters;
Data Presentation; Effective Figure Design and Legends
using the Gronlien article as a model.
|Homework: Study dilution making for Lab 3; |
Generate two figures with legends from your Tetrahymena experiment:
one or more microphotographs of Tetrahymena and a graph or table of data. Generate a figure of means +/- standard deviations and a caption for the theoretical data table at the end of the Assignments section in lab 2.
|Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena;
Design your own experiment exploring
inhibitors and Tetrahymena phagocytosis
|Homework: There are animated tutorials on dilution|
available at the following link:
Dilution Help Tutorials;
Finalize your experimental design for Lab 4; have it approved by your lab instructor;
Write a results section of a scientific report
on your findings in the Tetrahymena toxicity experiment
including a narrative portion and integrated figure(s) with legend(s);
Solve the dilution problems found at
Media: Lab_3_110_Dilution_Problems.doc and hand them in
by the beginning of Lab 4.
|Student Designed Experiments:
Potential Inhibitors of Vacuole Formation;
|Homework: 5 minute oral presentation in PowerPoint™ of your data,|
including at least one figure with legend, of your Lab 4 experimental results.
Read assigned journal article for science writing discussion
* Gronlien, H.K., Berg, T., Lovlie, A.M. (2002).
In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes
to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097
|Scientific Writing Workshop with Journal Article Discussion
||Homework: Tetrahymena Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6|
Title Page, Results, Discussion and References sections.
Guidelines at: Resources;
Science Writing peer-tutor appointment scheduling at:
Wellesley College Writing Program Resources.
Have Lab notebooks ready to be turned in for inspection after Lab 6.
Read Lab 6 (first lab in Series 2, Genetics)
Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
|Genetics: Taster SNP 1-Test PTC Taster Phenotype;
Isolate and digest DNA; PCR;
|Homework: Read the following background information on|
and on gel electrophoresis
Read the following background information on ethical issues in genetic testing:
Direct-to-Consumer Genetic Testing,
Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18.
A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,
published in Science, 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)
AMA DTC testing policy recommendations
Draw the expected migration locations of the Fnu4H1 digested PCR product
obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)
and a heterozygote (right lane) on a diagram of an agarose gel;
Answer the questions found at:
to use as a template for your answers.
|Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;
Determine PTC Taster Genotype; Scientific Writing:
Introduction & Discussion
Prepare draft figures/tables from the preliminary course data
for the taster investigation paper and an skeleton outline
of your introduction & discussion sections.
(Refer to the handout on How to Prepare a Skeleton Outline in Sakai.)
|2 & 3
|Data Analysis of Taster investigation; Science Writing Workshop:
Effective Figure Design, Introduction, Discussion; Photosynthesis 1:
Chlorophyll pigment investigation, Spectroscopy,
The Beer-Lambert Law and Creating a Standard Curve
|Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.|
For help, see the Resources section of this wiki.
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).
|Photosynthesis 2: Hill Reaction
||Homework: Finalize your self-designed experiment|
testing a variable affecting the Hill Reaction;
Write a Materials and Methods style version of the Hill Reaction
experiment that you did this week in Lab 9
|Photosynthesis 3: Investigation of variables affecting the Hill Reaction
||Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis)|
for the different treatments (with the treatments on the same graph).
Determine regression equations for the linear part of each data plots
to get slope (m) values (slopes represent the rates of electron transport).
Calculate the rates (change over time of the linear portion of your graphs)
and plot those values against your variable (x-axis);
A full paper on your experiment, in the form of a scientific research report,
is due at beginning of Lab 11 (or on day indicated in Lab Calendar).
Discuss your results in terms of how the factor that you chose to investigate
affects the electron transport rate in photosynthesis.
Compare your results to other investigations published in the primary literature.
Refer to the " Guidelines for science writing" information
found in the Resources section of the wiki.
|Science Writing Workshop
Bring a draft of your Results & Discussion sections (5 points)
(including figures/tables) of your photosynthesis paper
|Homework: Prepare for a Lab Practical Skills Assessment in lab 12|
Make sure Lab Notebook is ready to turn in for grading at the beginning of LAB 12
Turn in Lab Notebook
|End of lab