BISC110/S12:Schedule: Difference between revisions
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| Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br> | | Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br> | ||
| '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/ | | '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/S12:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br> | ||
Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena''<BR> from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'',<BR> the non-selective phagocytosis seen in microstomes <BR>changes to a highly selective process in macrostomes.<BR> (2002), Journal of Experimental Biology 205, 2089-2097; <BR> | Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena''<BR> from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'',<BR> the non-selective phagocytosis seen in microstomes <BR>changes to a highly selective process in macrostomes.<BR> (2002), Journal of Experimental Biology 205, 2089-2097; <BR> | ||
'''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin,<BR> and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257. | '''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin,<BR> and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257. | ||
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| Scientific Writing Workshop with Journal Article Discussion | | Scientific Writing Workshop with Journal Article Discussion | ||
| '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/ | | '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/S11:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: <BR>[http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br> | ||
Have Lab notebooks ready to be turned in for inspection after Lab 6. | Have Lab notebooks ready to be turned in for inspection after Lab 6. | ||
<br> Read Lab 6 (first lab in Series 2, Genetics) | <br> Read Lab 6 (first lab in Series 2, Genetics) | ||
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[http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br> | [http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br> | ||
Draw the expected migration locations of the Fnu4H1 digested PCR product<BR> obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br> | Draw the expected migration locations of the Fnu4H1 digested PCR product<BR> obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br> | ||
Answer the questions found at:<BR>[[Media:Taster_DTC_questions110.doc]] <BR>to use as a template for your answers.''' | Answer the questions found at:<BR><font color="red">[[Media:Taster_DTC_questions110.doc]] </font color="red"><BR>to use as a template for your answers.''' | ||
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| Data Analysis of Taster investigation; Science Writing Workshop:<BR> Effective Figure Design, Introduction, Discussion; Photosynthesis 1:<BR> Chlorophyll pigment investigation, Spectroscopy,<BR> The Beer-Lambert Law and Creating a Standard Curve | | Data Analysis of Taster investigation; Science Writing Workshop:<BR> Effective Figure Design, Introduction, Discussion; Photosynthesis 1:<BR> Chlorophyll pigment investigation, Spectroscopy,<BR> The Beer-Lambert Law and Creating a Standard Curve | ||
| '''Homework''': Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.'''<BR> For help, see the [[BISC110/ | | '''Homework''': Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.'''<BR> For help, see the [[BISC110/S12:Resources | Resources]] section of this wiki.<br> | ||
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br> | In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br> | ||
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br> | Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br> | ||
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| Photosynthesis 3: Investigation of variables affecting the Hill Reaction | | Photosynthesis 3: Investigation of variables affecting the Hill Reaction | ||
| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments (with the treatments on the same graph).<BR>See([[Appendix_E/ | | '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments (with the treatments on the same graph).<BR>See([[Appendix_E/S12 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots<BR> to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs)<BR> and plot those values against your variable (x-axis);<br> | ||
'''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11 (or on day indicated in Lab Calendar).'''<BR> Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/ | '''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11 (or on day indicated in Lab Calendar).'''<BR> Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/S12:Guidelines for Science Writing | '''Guidelines for science writing''']]" information<BR> found in the [[BISC110/S12:Resources | Resources]] section of the wiki. | ||
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Revision as of 12:21, 2 December 2011
BISC110/112 S12 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday |
---|---|---|---|---|
Jan.23 | Jan. 24 |
Jan. 25 Classes begin |
Jan. 26 | Jan. 27 |
Jan. 30 | Jan. 31 Lab |
Feb. 1 Lab |
Feb. 2 | Feb. 3 |
Feb. 6 | Feb. 7 Lab |
Feb. 8 Lab |
Feb. 9 | Feb. 10 |
Feb. 13 | Feb. 14 Lab |
Feb. 15 Lab |
Feb. 16 | Feb. 17 |
Feb. 20 Presidents' Day |
Feb. 21 Lab |
Feb. 22 Lab |
Feb. 23 Monday Schedule |
Feb. 24 |
Feb. 27 | Feb. 28 Lab |
Feb. 29 Lab |
Mar. 1 | Mar. 2 |
Mar. 5 | Mar. 6 Lab |
Mar. 7 Lab |
Mar. 8 | Mar. 9 |
Mar. 12 | Mar. 13 Lab |
Mar. 14 Lab |
Mar. 15 | Mar. 16 |
Mar. 19 Spring Break |
Mar. 20 Spring Break |
Mar. 21 Spring Break |
Mar. 22 Spring Break |
Mar. 23 Spring Break |
Mar. 26 | Mar. 27 Lab |
Mar. 28 Lab |
Mar. 29 | Mar. 30 |
Apr. 2 | Apr. 3 Lab |
Apr. 4 Lab |
Apr. 5 | Apr. 6 |
Apr. 9 | Apr. 10 Lab |
Apr. 11 Lab |
Apr. 12 | Apr. 13 |
Apr. 16 Patriots' Day |
Apr. 17 Lab 1 |
Apr. 18 Lab |
Apr. 19 | Apr. 20 Monday Schedule |
Apr. 23 | Apr. 24 |
Apr. 25 Rhulman NO Lab |
Apr. 26 | April 27 |
Apr. 30 | May 1 Lab |
May 2 Lab |
May 3 | May 4 Last day of classes |
Schedule of Experiments
Section | Lab # | Activities |
---|---|---|
1 | 1-5 | Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing |
2 | 6-8 | Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis; Scientific Writing & Effective figure design |
3 | 8-11 | Photosynthesis: Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems |
Lab Practical | 12 | Hands-on skills assessment: |
BISC110/112 Assignments/ Weekly Lab Planner
Series | Lab # | LAB DATES | LAB DESCRIPTION | Assignment/Notes | |
1 | 1 | to |
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis |
Homework: Familiarize yourself with the wiki, particularly the information in the BISC110/S12:Resources section of the Lab wiki; Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_Problems110.doc; Read about the process of endocytosis in your textbook; | |
1 | 2 | to |
Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. |
Homework: Study dilution making for Lab 3; Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data. | |
1 | 3 | to |
Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis |
Homework: There are animated tutorials on dilution available at the following link: Dilution Help Tutorials; Finalize your experimental design for Lab 4; have it approved by your lab instructor; Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figure(s) with legend(s); Solve the dilution problems found at | |
1 | 4 | to |
Student Designed Experiments: Potential Inhibitors of Vacuole Formation; |
Homework: 5 minute oral presentation in PowerPoint™ of your data, including at least one figure with legend, of your Lab 4 experimental results. Read assigned journal article for science writing discussion * Gronlien, H.K., Berg, T., Lovlie, A.M. (2002). In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097 | |
1 | 5 | to |
Scientific Writing Workshop with Journal Article Discussion | Homework: Tetrahymena Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6 Title Page, Results, Discussion and References sections. Guidelines at: Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources. Have Lab notebooks ready to be turned in for inspection after Lab 6.
| |
2 | 6 | to |
Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; |
Homework: Read the following background information on restriction enzymes and on gel electrophoresis Read the following background information on ethical issues in genetic testing: | |
2 | 7 | to |
Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis; Determine PTC Taster Genotype; Scientific Writing: Introduction & Discussion |
Homework:
Prepare draft figures/tables from the preliminary course data | |
2 & 3 | 8 | to |
Data Analysis of Taster investigation; Science Writing Workshop: Effective Figure Design, Introduction, Discussion; Photosynthesis 1: Chlorophyll pigment investigation, Spectroscopy, The Beer-Lambert Law and Creating a Standard Curve |
Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study. For help, see the Resources section of this wiki. In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9); | |
3 | 9 | to |
Photosynthesis 2: Hill Reaction | Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction; Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | |
3 | 10 | to |
Photosynthesis 3: Investigation of variables affecting the Hill Reaction | Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (with the treatments on the same graph). See(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis); A full paper on your experiment, in the form of a scientific research report, | |
3 | 11 | to |
Science Writing Workshop Bring a draft of your Results & Discussion sections (5 points) (including figures/tables) of your photosynthesis paper |
Homework: Prepare for a Lab Practical Skills Assessment in lab 12
| |
Skills Assessment | 12 | to |
Lab Practical; Turn in Lab Notebook |
End of lab |
Lab Graded Assignments
Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
---|---|---|---|
1 | 2 | Practice Problems | 5 |
2 | 3 | Fig. Design | 5 |
3 | 4 | Dilution Problems (5); Fig.& Results (10) |
15 |
4 | 5 | Oral presentation: Results, original figure & legend; Journal article discussion |
10 |
5 | 6 | Lab notebook check (ungraded); Partial Scientific Paper(25) |
25 |
6 | 7 | SNP Taster Questions | 5 |
7 | 8 | Draft figures/tables & outline of Taster paper | 5 |
8 | 9 | Taster Scientific Paper (all but M&M) | 35 |
9 | 10 | M&M (5pt); Finalize Self-Design Experiment Hill Rxn.(ungraded) |
5 |
10 | 11 | Draft Results & Discussion for Science Writing Workshop | 5 |
11 | 12 (or on day indicated in calendar) | Full Scientific Paper: Factor Affecting Rate of Photosynthesis | 35 |
12 | 12 | Lab Notebook Check (10) Lab Practical:Hands-on skills assessment (35) |
45 |
Other | - | Discretionary Points Preparation & Participation |
5 |