BISC110/S11:Schedule: Difference between revisions
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=='''Schedule of Experiments'''== | =='''Schedule of Experiments'''== | ||
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| Feb. 14 to <br> Feb. 18 | | Feb. 14 to <br> Feb. 18 | ||
| Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation; | | Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation; | ||
| '''Homework''': 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR> | | '''Homework''': 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>'''* Gronlien, H.K., Berg, T., Lovlie, A.M.''' (2002). In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097 | ||
|-- | |-- | ||
| 1 | | 1 | ||
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| Feb. 22 to <br> Feb. 28 | | Feb. 22 to <br> Feb. 28 | ||
| Scientific Writing Workshop with Journal Article Discussion;<BR> Science Writing Workshop; | | Scientific Writing Workshop with Journal Article Discussion;<BR> Science Writing Workshop; | ||
| '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/ | | '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/S11:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: [http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br> | ||
Have Lab notebooks ready to be turned in | Have Lab notebooks ready to be turned in for inspection after Lab 6. | ||
<br> Read Lab 6 (first lab in Series 2, Genetics) | <br> Read Lab 6 (first lab in Series 2, Genetics) | ||
<br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures. | <br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures. | ||
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| March 1 to<br> March 7 | | March 1 to<br> March 7 | ||
| Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR; | | Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR; | ||
| '''Homework''': Read the following background information | | '''Homework''': Read the following background information on [http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf restriction enzymes] and on [http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf gel electrophoresis] <br> | ||
and on | Read the following background information on ethical issues in genetic testing:<br> | ||
Read the following background information | '''[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710700/?tool=pubmed Direct-to-Consumer Genetic Testing,''' Two Sides of the Coin] by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18. <BR> | ||
Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 | [http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf PUBLIC HEALTH: A Case Study of Personalized Medicine] by S. H. Katsanis, G. Javitt, and K. Hudson, published in ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)<BR> | ||
PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in | [http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br> | ||
''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum) | Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br> | ||
Answer the questions found at:<BR>[[Media:Taster_DTC_questions110.doc]] to use as a template for your answers.''' | |||
Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; | |||
<br>Answer the questions found at | |||
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
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| 7 | | 7 | ||
| March 8 to <br> March 14 | | March 8 to <br> March 14 | ||
| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ;<BR> Determine PTC Taster Genotype; Scientific Writing Workshop | | Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;<BR> Determine PTC Taster Genotype; Scientific Writing Workshop | ||
| '''Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.<BR> Due at | | '''Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.<BR> Due on April 1st at 5pm.'''<BR> For help, see the [[BISC110/S11:Resources | Resources]] section of this wiki. | ||
|-- | |-- | ||
| 3 | | 3 | ||
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| March 28 to<br> April 1 | | March 28 to<br> April 1 | ||
| Photosynthesis 1: Photosynthetic pigments | | Photosynthesis 1: Photosynthetic pigments | ||
| '''Homework''': Prepare a full results section | | '''Homework''': Prepare a full results section for the photosynthetic pigment identification experiment;<br> | ||
In your lab notebook, | In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br> | ||
Review the mechanisms of electron transport | Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br> | ||
|-- | |-- | ||
| 3 | | 3 | ||
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| April 4 to<br> April 8 | | April 4 to<br> April 8 | ||
| Photosynthesis 2: Hill Reaction | | Photosynthesis 2: Hill Reaction | ||
| '''Homework''': Finalize your self-designed experiment | | '''Homework''': Finalize your self-designed experiment testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | ||
|-- | |-- | ||
| 3 | | 3 | ||
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| April 11 to <br> April 15 | | April 11 to <br> April 15 | ||
| Photosynthesis 3: Investigation of variables affecting the Hill Reaction | | Photosynthesis 3: Investigation of variables affecting the Hill Reaction | ||
| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis) | | '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (with the treatments on the same graph).See([[Appendix_E/S11 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis);<br> | ||
'''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11.'''<BR> Discuss your results in terms of how the factor that you chose to investigate | '''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11.'''<BR> Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/S11:Guidelines for writing a lab report in the form of a scientific paper | '''Guidelines for science writing''']]" information found in the [[BISC110/S11:Resources | Resources]] section of the wiki. | ||
|-- | |-- | ||
| 4 | | 4 | ||
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| Enzymology | | Enzymology | ||
| '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12.'''<br> | | '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12.'''<br> | ||
Answer the following questions | Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. <BR>Download a Word document form of these questions here:[[Media: Lab_11_Questions_110.doc]] | ||
<br> Make sure Lab Notebook is ready to turn in for grading | <br> Make sure Lab Notebook is ready to turn in for grading | ||
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|- | |- | ||
! 7 | ! 7 | ||
| Due for all students<BR> Fri. 4/ | | Due for all students<BR> Fri. 4/1 by 5pm | ||
| Taster Scientific Paper (all but M&M) | | Taster Scientific Paper (all but M&M) | ||
| 35 | | 35 |
Latest revision as of 14:04, 5 January 2012
BISC110/112 Spring 2011 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday | |
---|---|---|---|---|---|
January 24 Lab 1 |
January 25 Lab 1 |
January 26 Lab 1 |
January 27 Lab 1 |
January 28 Lab 1 |
|
January 31 Lab 2 |
February 1 Lab 2 |
February 2 Lab 2 |
February 3 Lab 2 |
February 4 Lab 2 |
|
February 7 Lab 3 |
February 8 Lab 3 |
February 9 Lab 3 |
February 10 Lab 3 |
February 11 Lab 3 |
|
February 14 Lab 4 |
February 15 Lab 4 |
February 16 Lab 4 |
February 17 Lab 4 |
February 18 Lab 4 |
|
February 21 President's Day NO LAB |
February 22 Lab 5 |
February 23 Lab 5 |
February 24 Lab 5 |
February 25 Lab 5 |
|
February 28 Lab 5 |
March 1 Lab 6 |
March 2 Lab 6 |
March 3 Lab 6 |
March 4 Lab 6 |
|
March 7 Lab 6 |
March 8 Lab 7 |
March 9 Lab 7 |
March 10 Lab 7 |
March 11 Lab 7 |
|
March 14 Lab 7 |
March 15 NO LAB |
March 16 NO LAB |
March 17 NO LAB Spring Break |
March 18 NO LAB Spring Break |
|
March 21 Spring Break |
March 22 Spring Break |
March 23 Spring Break |
March 24 Spring Break |
March 25 Spring Break |
|
March 28 Lab 8 |
March 29 Lab 8 |
March 30 Lab 8 |
March 31 Lab 8 |
April 1 Lab 8 |
|
April 4 Lab 9 |
April 5 Lab 9 |
April 6 Lab 9 |
April 7 Lab 9 |
April 8 Lab 9 |
|
April 11 Lab 10 |
April 12 Lab 10 |
April 13 Lab 10 |
April 14 Lab 10 |
April 15 Lab 10 |
|
April 18 Patriots' Day NO LAB |
April 19 Monday Schedule NO LAB |
April 20 Lab 11 |
April 21 Lab 11 |
April 22 Lab 11 |
|
April 25 Lab 11 |
April 26 Lab 11 |
April 27 Rhulman NO LAB |
April 28 NO LAB |
April 29 NO LAB |
|
May 2 Lab 12 |
May 3 Lab 12 |
May 4 Lab 12 |
May 5 Lab 12 |
May 6 Lab 12 |
Schedule of Experiments
Section | Lab # | Activities |
---|---|---|
1 | 1-5 | Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing |
2 | 6-7 | Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis |
3 | 8-10 | Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems |
4 | 11 | Gene Regulation/Enzymology: Invertase enzyme assay |
Lab Practical | 12 | Hands-on skills assessment: |
BISC110/112 Assignments/ Weekly Lab Planner
Series | Lab # | LAB DATES | LAB DESCRIPTION | Assignment/Notes | ||
1 | 1 | Jan. 24 to Jan. 28 |
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis |
Homework: Familiarize yourself with the wiki, particularly the information in the BISC110/S11:Resources section of the Lab wiki; Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_Problems110.doc; Read about the process of endocytosis in your textbook; familiarize yourself with phagocytosis in Tetrahymena from the following reference articles: | ||
1 | 2 | Jan. 31 to Feb. 4 |
Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. | Homework: Study dilution making for Lab 3; Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data. | ||
1 | 3 | Feb. 7 to Feb. 11 |
Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis |
Homework: There are animated tutorials on dilution available at the following link: Dilution Help Tutorials; Finalize your experimental design for Lab 4; have it approved by your lab instructor; Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends; Work the dilution problems found at Media: Lab_3_110_Dilution_Problems.doc and hand them in in Lab 4. | ||
1 | 4 | Feb. 14 to Feb. 18 |
Student Designed Experiments: Potential Inhibitors of Vacuole Formation; |
Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. Read assigned journal article for science writing discussion * Gronlien, H.K., Berg, T., Lovlie, A.M. (2002). In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097 | ||
1 | 5 | Feb. 22 to Feb. 28 |
Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop; |
Homework: Tetrahymena Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6 Title Page, Results, Discussion and References sections. Guidelines at: Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources. Have Lab notebooks ready to be turned in for inspection after Lab 6.
| ||
2 | 6 | March 1 to March 7 |
Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; |
Homework: Read the following background information on restriction enzymes and on gel electrophoresis Read the following background information on ethical issues in genetic testing: | ||
2 | 7 | March 8 to March 14 |
Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis; Determine PTC Taster Genotype; Scientific Writing Workshop |
Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study. Due on April 1st at 5pm. For help, see the Resources section of this wiki. | ||
3 | 8 | March 28 to April 1 |
Photosynthesis 1: Photosynthetic pigments | Homework: Prepare a full results section for the photosynthetic pigment identification experiment; In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9); | ||
3 | 9 | April 4 to April 8 |
Photosynthesis 2: Hill Reaction | Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction; Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | ||
3 | 10 | April 11 to April 15 |
Photosynthesis 3: Investigation of variables affecting the Hill Reaction | Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (with the treatments on the same graph).See(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis); A full paper on your experiment, in the form of a scientific research report, | ||
4 | 11 | April 20 to April 26 |
Enzymology | Homework: Prepare for a Lab Practical Skills Assessment in lab 12. Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. | ||
Skills Assessment | 12 | May 2 to May 6 |
Lab Practical; Turn in Lab Notebook |
End of lab |
Lab Graded Assignments
Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
---|---|---|---|
1 | 2 | Practice Problems | 5 |
2 | 3 | Fig. Design (ungraded) | 0 |
3 | 4 | Dilution Problems (5); Fig.& Results (10) |
15 |
4 | 5 | Oral presentation: Results, original figure & legend; Journal article discussion |
10 |
5 | 6 | Lab notebook check (ungraded); Partial Scientific Paper(25) |
25 |
6 | 7 | SNP Taster Questions | 5 |
7 | Due for all students Fri. 4/1 by 5pm |
Taster Scientific Paper (all but M&M) | 35 |
8 | 9 | Photosyn. Pigments Results | 10 |
9 | 10 | M&M (5pt); Finalize Self-Design Experiment Hill Rxn.(ungraded) |
5 |
10 | 11 | Full Scientific Paper Hill Rxn. | 35 |
11 | 12 | Lab Notebook Check (10) Concept Questions Enzymology/Gene Reg. (5) |
15 |
12 | 12 | Lab Practical:Hands-on skills assessment |
35 |
Other | - | Discretionary Points Preparation & Participation |
5 |