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Wellesley College-BISC110 Introduction to Cell Biology-Fall 2009

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BISC110 F09 Lab Calendar

Monday Tuesday Wednesday Thursday Friday
Sept. 7
Labor Day Holiday
Sept. 8
Lab 1
Sept. 9
Lab 1
Sept. 10
Lab 1
Sept. 11
Lab 1
Sept. 14
Lab 1
Sept. 15
Lab 2
Sept. 16
Lab 2
Sept. 17
Lab 2
Sept. 18
Lab 2
Sept. 21
Lab 2
Sept. 22
Lab 3
Sept. 23
Lab 3
Sept. 24
Lab 3
Sept. 25
Lab 3
Sept. 28
Lab 3
Sept. 29
Lab 4
Sept. 30
Lab 4
Oct. 1
Lab 4
Oct. 2
Lab 4
Oct. 5
Lab 4
Oct. 6
Lab 5
Oct. 7
Lab 5
Oct. 8
Lab 5
Oct. 9
Lab 5
Oct. 12
Fall Break
No Lab
Oct. 13
No Lab
Oct. 14
Wed. follows Mon.

Lab 5
Oct. 15
No Lab
Oct. 16
No Lab
Oct. 19
Lab 6
Oct. 20
Lab 6
Oct. 21
Lab 6
Oct. 22
Lab 6
Oct. 23
Lab 6
Oct. 26
Lab 7
Oct. 27
Lab 7
Oct. 28
Lab 7
Oct. 29
Lab 7
Oct. 30
Lab 7
Nov. 2
Lab 8
Nov. 3
Tanner Conference
No Lab
Nov. 4
Lab 8
Nov. 5
Lab 8
Nov. 6
Lab 8
Nov. 9
No Lab
Nov. 10
Lab 8
Nov. 11
Lab 9
Nov. 12
Lab 9
Nov. 13
Lab 9
Nov. 16
Lab 9
Nov. 17
Lab 9
Nov. 18
Lab 10
Nov. 19
Lab 10
Nov. 20
Lab 10
Nov. 23
Lab 10
Nov. 24
Lab 10
Nov. 25
No Lab
Nov. 26
Thanksgiving Holiday
Nov. 27
Thanksgiving Holiday
No Lab
Nov. 30
Lab 11
Dec. 1
Lab 11
Dec. 2
Lab 11
Dec. 3
Lab 11
Dec. 4
Lab 11
Dec. 7
Lab 12
Dec. 8
Lab 12
Dec. 9
Lab 12
Dec. 10
Lab 12
Dec. 11
Lab 12

Schedule of Experiments

Section Lab # Activities
1 1-4 Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing
2 5-7 Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis; Case study, ethics
3 8-10 Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems
4 11 Gene Regulation/Enzymology: Invertase enzyme assay
Lab Practical 12 Hands-on skills assessment:


BISC110 Assignments/ Weekly Lab Planner

Series Lab # DATE LAB DESCRIPTION Assignment/Notes
1 1 Tues. 9/9-
Mon. 9/14
Part 1: Practice metric conversions;
Part 2: Practice pipetting;
Part 3: Making Solutions;
Part 4: Microscopy;
Part 5: Examination of Tetrahymena pyriformis
Part 6: Making Dilutions;
Part 7: Determination of Non-toxic Concentration of Solutions
Homework: Familiarize yourself with the information in the BISC110/F09:Resources section of the Lab wiki;
Lab 1 Practice Problems downloadable here Media:Practice_Problems_Lab 1.doc

There are animated tutorials available at the following link: Dilution Help Tutorials

1 2 Tue. Sept. 15-
Mon. Sept 21
Part 1: Tetrahymena Behavior; Preparation of Ink Solutions; How Scientists Work-Observations & Hypothesis Generation;
Part 2: Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation
Part3: Group Discussion & Hypothesis Generation
Homework: Finalize your experimental design for Lab 3 and email a detailed protocol to your instructor well before the next lab;
Generate two figures with legends from your ink concentration experiment: one of one or more photographs of Tetrahymena and the other a graph of the data.
1 3 Tues. Sept. 22-
Mon. Sept. 28
Part 1: Student Designed Experiments: Potential Inhibitors of Vacuole Formation Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your lab 3 experimental results.
Read assigned journal article: Chiesa R., Silva W., Renaud F., Pharmacological characteristics of an opoid receptor in the ciliate Tetrahymena. J. Euk. Microbiol. 406(6) 1993, 800-804.[Link to Wellesley College Library]
1 4 Tues. Sept. 29-
Oct. 5
Part 1: Scientific Writing Workshop with Journal Article Discussion
Part 2: Figure Presentation Workshop
Part 3: Finding Sources
Homework: Partial lab report: Title Page, Results, Discussion and References sections. Guidelines at:BISC110/F09:Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources.

Have Lab notebooks ready to be turned in for inspection after Lab 5.
Read Lab 5 (first lab in Series 2, Genetics)
Prepare a flow chart in your lab notebook of all Lab 5 experimental procedures.

2 5 Tues. Oct. 6-
Wed. Oct. 14
Genetics: Taster SNP 1 Homework: Read the following background information on restriction enzymes found at:(

and on gel electrophoresis at:
(; also Read the following background information for a case study on ethical issues in genetic testing: Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 and found at: (; PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in Science, 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604] (in Policy Forum) found at: (; AMA DTC testing policy recommendations at: (; Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel; Answer the questions found at the following downloadable link:

2 6 Mon. Oct. 19-
Fri. Oct. 23
Genetics: Taster SNP 2 Homework: Prepare a formal figure of the gel picture you obtained today, complete with title, legend and labels. For help, see the Resources section of this wiki: " Guidelines for science writing";

Prepare for your lab group Oral Presentation on a DTC company. For help, see the Resources section of this wiki: Guidelines for Oral Presentations.

2 7 Mon. Oct. 26-
Fri. Oct. 30
Genetics: Taster SNP 3 Homework:
3 8 Mon. Nov. 2-
Tues. Nov. 10
Photosynthesis 1: Photosynthetic pigments Homework: Media:Assignment for Lab 8.doc Download Questions (Answers to be turned in next lab) through this link;

Label your printed spectra and chromatogram, and write figure legends for them;
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).

3 9 Wed. Nov. 11-
Tues. Nov. 17
Photosynthesis 2: Hill Reaction Homework: E-mail your self-designed experiment testing a variable affecting the Hill Reaction to your lab instructor (cc your partner) by your instructor's deadline for first submission;
Modify the proposal after receiving your instructor's suggestions and resubmit it by your instructor's deadline;
Proposals must include:– your hypothesis, controls and expected results as well as your protocol (with sufficient detail, e.g., how to set up your tubes, etc).
3 10 Wed. Nov. 18-
Tues. Nov. 24
Photosynthesis 3: Investigation of variables affecting the Hill Reaction Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (you can plot several or all data sets on one graph). Plot all data on the same graph as regression lines for ease of comparison (Appendix E). Include the regression equations. The slopes represent the rates of the Hill reactions. Do not include points that have leveled off. Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis).

A complete Lab Report in the form of a scientific research report on your experiment today is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate and the coupling of electron transport with the photophosphorylation of ADP and/or the role of the H+ gradient in these processes. Compare your results to other investigations published in the primary literature. Refer to the " Guidelines for science writing" information found in the Resources section of the wiki.

4 11 Mon. Nov. 30-
Fri. Dec 4
Enzymology Homework: Prepare for a Lab Practical Skills Assessment and return the Take Home Quiz in Lab 12.

Answer the following questions (10 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions.doc
Make sure Lab Notebook is ready to turn in for grading

Skills Assessment 12 Mon. Dec. 7-
Fri. Dec 11
Lab Practical; Turn in Take Home Quiz; Turn in Lab Notebook End of lab

Lab Graded Assignments

Lab Assigned Lab Due Assignment
Point Value
(Total 200)
1 2 Practice Problems 5
2 3 Fig. Design Ungraded
3 4 Oral presentation:original figure & legend;
Journal article discussion
4 5 Partial Scientific Paper 25
5 6 Lab notebook check (ungraded)  ?
6 7  ?  ?
7 8  ?  ?
8 9 Photosyn. Pigments Questions 5
9 10 Design Experiment Hill Rxn. Ungraded
10 11 Full Lab Report Hill Rxn. 35
11 12 Lab Notebook Check (10)
Concept Questions Enzymology/Gene Reg. (10)
12 12 Lab Practical:Hands-on skills assessment
& Take Home Quiz: Concepts evaluation:
Other - Discretionary Points
Preparation & Participation