BE.109:Bio-material engineering

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BE.109 Laboratory Fundamentals of Biological Engineering

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DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       

Module 4

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

Lab handouts

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6