Difference between revisions of "BE.109:Bio-material engineering"

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==Lab handouts==
==Lab handouts==
'''[[BE.109:Bio-material engineering/Panning for gold binders | Day 1: Screening library]]'''
'''[[BE.109:Bio-material engineering/Screening library | Day 1: Screening library]]'''
'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 2: Optimizing panning]]'''
'''[[BE.109:Bio-material engineering/Optimizing panning | Day 2: Optimizing panning]]'''
'''[[BE.109:Bio-material engineering/Checking gold binders | Day 3: Rescreening gold binders]]'''
'''[[BE.109:Bio-material engineering/Rescreening gold binders | Day 3: Rescreening gold binders]]'''
'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 4: PCR of gold binding candidates]]'''
'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 4: PCR of gold binding candidates]]'''

Revision as of 19:56, 5 February 2006

BE.109 Laboratory Fundamentals of Biological Engineering

BE LongImage-1.jpg

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DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       

Module 4

Instructors: Angela Belcher and Natalie Kuldell

TA: Eileen Higham

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

S. cerevisiae shown budding (top) and bound to CdS (bottom)

Lab handouts

Day 1: Screening library

Day 2: Optimizing panning

Day 3: Rescreening gold binders

Day 4: PCR of gold binding candidates

Day 5: Student presentations

Day 6: Sequence analysis


Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.

  1. Design criteria for engineering inorganic material-specific peptides.
    Langmuir 2005 21(15):6929-6933
    Peelle BR, Krauland EM, Wittrup KD, Belcher AM
    URL PDF reprint first supplement to pdf second supplement to pdf