BCH072506-Construction of pSB3C6-J23018
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[p15A]+ E [TT][Ptet][RBS] X [RFP] S P
Digest pSB1A2-J23018 (EcoRI/PstI, EcoRI buffer, small-935bp)
Paste into pSB3C6 (EcoRI/PstI, EcoRI buffer, large-2028bp)
Product is pSB3C6-J23028
[p15A]+ E [TT][Ptet][RBS] X [RFP] S P
Digest pSB1A2-J23018 (EcoRI/PstI, EcoRI buffer, small-935bp)
Paste into pSB3C6 (EcoRI/PstI, EcoRI buffer, large-2028bp)
Product is pSB3C6-J23028