Antarctic Phosphatase

From OpenWetWare
Revision as of 18:34, 18 August 2011 by David Gorczyca (talk | contribs) (Notes)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search


NEB's Antarctic Phosphatase

Associated protocols

Phosphatase treatment of linearized vector


This enzyme is preferable to either Calf Intestinal Phosphatase or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.