Annexin-V

• Conjugated to other fluorochromes (PE, FITC)
• Stains cells in early stage of apoptosis
  • Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
• Must be used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)