Difference between revisions of "Annexin-V"

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(New page: * Conjugated to other fluorochromes (PE, FITC) * Stains cells in '''early stage of apoptosis''' ** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane...)
 
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* Stains cells in '''early stage of apoptosis'''
 
* Stains cells in '''early stage of apoptosis'''
 
** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
 
** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
 +
* Must be used in conjunction with vital dye (e.g. [[Propidium iodide|PI]], [[7-AAD]]) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)

Revision as of 13:19, 6 July 2008

  • Conjugated to other fluorochromes (PE, FITC)
  • Stains cells in early stage of apoptosis
    • Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
  • Must be used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)