Altman:Protocols/TC Protocols/ARPE-19 conditions: Difference between revisions
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David Altman (talk | contribs) (New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Maintaining ARPE-19 cells== * ARPE-19 cells are grown on the surface of a T-25 flask * Cells ...) |
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Latest revision as of 12:29, 24 July 2013
Maintaining ARPE-19 cells
- ARPE-19 cells are grown on the surface of a T-25 flask
- Cells are maintained at 37 C and 5% CO2 in DMEM/F12 + 10% FBS + 100x pen-strep-glutamine + 10 mM HEPES (pH 7.4)
- When cells have reached 80% confluence, they are diluted and split into new flasks