Altman:Protocols/Single Molecule Assays/gliding filament: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
David Altman (talk | contribs) No edit summary |
David Altman (talk | contribs) No edit summary |
||
| Line 5: | Line 5: | ||
==Making Nitrocellulose Coverslips== | ==Making Nitrocellulose Coverslips== | ||
''Typical flow-through volumes are 10-15 μL'' | |||
1. Make a flow cell with a nitrocellulose-coated coverslip | 1. Make a flow cell with a nitrocellulose-coated coverslip | ||
| Line 20: | Line 20: | ||
Recipes | '''Recipes''' | ||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | <table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | ||
Revision as of 13:49, 15 June 2013
Department of Physics, Willamette University
Making Nitrocellulose Coverslips
Typical flow-through volumes are 10-15 μL
1. Make a flow cell with a nitrocellulose-coated coverslip 2. Flow in α-GFP/10 3. Incubate 2 minutes 4. Flow in ABSA 5. Incubate 2 minutes 6. Flow in motor dilution 7. Incubate 2 minutes 8. Flow in ABSA 9. Flow in Actin dilution 10. Incubate 2 minutes 11. Flow in GO-Juice
Recipes
| 50 uL Buffer #1 | |
|---|---|
| 50x GOC stock | 2 μL |
| 100x CPK stock | 1 μL |
| 100x ATP stock | 1 μL |
| AB | 46 μL |
| 50 μL Buffer #2 | |
|---|---|
| 50x Glu/B stock | 2 μL |
| 100x PCr stock | 1 μL |
| AB | 47 μL |
| 20 μL GO Juice | |
|---|---|
| Buffer #1 | 10 μL |
| Buffer #2 | 10 μL |
