Altman:Protocols/Single Molecule Assays/gliding filament: Difference between revisions
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==Making Nitrocellulose Coverslips== | ==Making Nitrocellulose Coverslips== | ||
//Typical flow-through volumes are 10-15 μL// | |||
1. Make a flow cell with a nitrocellulose-coated coverslip | 1. Make a flow cell with a nitrocellulose-coated coverslip | ||
Revision as of 13:48, 15 June 2013
Department of Physics, Willamette University
Making Nitrocellulose Coverslips
//Typical flow-through volumes are 10-15 μL//
1. Make a flow cell with a nitrocellulose-coated coverslip 2. Flow in α-GFP/10 3. Incubate 2 minutes 4. Flow in ABSA 5. Incubate 2 minutes 6. Flow in motor dilution 7. Incubate 2 minutes 8. Flow in ABSA 9. Flow in Actin dilution 10. Incubate 2 minutes 11. Flow in GO-Juice
Recipes
| 50 uL Buffer #1 | |
|---|---|
| 50x GOC stock | 2 μL |
| 100x CPK stock | 1 μL |
| 100x ATP stock | 1 μL |
| AB | 46 μL |
| 50 μL Buffer #2 | |
|---|---|
| 50x Glu/B stock | 2 μL |
| 100x PCr stock | 1 μL |
| AB | 47 μL |
| 20 μL GO Juice | |
|---|---|
| Buffer #1 | 10 μL |
| Buffer #2 | 10 μL |
