Difference between revisions of "Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips"

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1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
 
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
 +
 
2. Spread 7.5 μL on each slide
 
2. Spread 7.5 μL on each slide
 +
 
3. Allow coverslips to dry
 
3. Allow coverslips to dry
 +
 
4. Dip coverslips in 0.1% collodion in amyl acetate
 
4. Dip coverslips in 0.1% collodion in amyl acetate
  
  
 
Recipes
 
Recipes
 
  
 
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">

Latest revision as of 17:11, 18 November 2012

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Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100

2. Spread 7.5 μL on each slide

3. Allow coverslips to dry

4. Dip coverslips in 0.1% collodion in amyl acetate


Recipes

2.5 mL 0.2% Triton
25% Triton X-100 20 μL
sterile water 2.5 mL


20 mL 0.1% Collodion
2% collodion in amyl acetate 1 mL
Amyl acetate 19 mL