Difference between revisions of "Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips"

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(New page: 1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 2. Spread 7.5 μL on each slide 3. Allow coverslips to dry 4. Dip coverslips in 0.1% collodion in amyl acetate Recipes...)
 
 
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==Making Nitrocellulose Coverslips==
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1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
 
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
 +
 
2. Spread 7.5 μL on each slide
 
2. Spread 7.5 μL on each slide
 +
 
3. Allow coverslips to dry
 
3. Allow coverslips to dry
 +
 
4. Dip coverslips in 0.1% collodion in amyl acetate
 
4. Dip coverslips in 0.1% collodion in amyl acetate
 +
  
 
Recipes
 
Recipes
0.2% Triton X-100
 
2.5 mL 0.2% Triton
 
25% Triton X-100 (lab fridge) 20 μL
 
Filtered water 2.5 mL
 
  
0.1 % Collodion in Amyl Acetate
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
20 mL 0.1% Collodion
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2% collodion in amyl acetate 1 mL
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<tr>
Amyl acetate 19 mL
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<th><tt> </tt></th>
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<th><tt>2.5 mL 0.2% Triton </tt></th>
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</tr>
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<tr>
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<th><tt> 25% Triton X-100 </tt></th>
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<th><tt> 20 μL </tt></th>
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</tr>
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<tr>
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<th><tt> sterile water </tt></th>
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<th><tt> 2.5 mL </tt></th>
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</tr>
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</table>
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
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 +
<tr>
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<th><tt> </tt></th>
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<th><tt> 20 mL 0.1% Collodion </tt></th>
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</tr>
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<tr>
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<th><tt> 2% collodion in amyl acetate </tt></th>
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<th><tt> 1 mL </tt></th>
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</tr>
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<tr>
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<th><tt> Amyl acetate </tt></th>
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<th><tt> 19 mL </tt></th>
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</tr>
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</table>

Latest revision as of 18:11, 18 November 2012

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Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100

2. Spread 7.5 μL on each slide

3. Allow coverslips to dry

4. Dip coverslips in 0.1% collodion in amyl acetate


Recipes

2.5 mL 0.2% Triton
25% Triton X-100 20 μL
sterile water 2.5 mL


20 mL 0.1% Collodion
2% collodion in amyl acetate 1 mL
Amyl acetate 19 mL