Altman:Protocols/Preparing Reagents/Making GB

Department of Physics, Willamette University

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Making Glucose Beta-Mercaptoethanol (G/β)

FINAL CONCENTRATION of 50x G/β stock:

• 22.5% (w/v) Glucose
• 12.5% (v/v) β-mercaptoethanol

Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

	1 mL G/β
Glucose	0.225 g
14.3 M β-mercaptoethanol	125 μL
1x AB	875 uL