Altman:Protocols/Preparing Reagents/Making GB

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Department of Physics, Willamette University

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Making Glucose Beta-Mercaptoethanol (G/β)

FINAL CONCENTRATION of 50x G/β stock:

22.5% (w/v) Glucose

12.5% (v/v) β-mercaptoethanol

in assay buffer

Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

1 mL G/β Glucose 0.225 g 14.3 M β-mercaptoethanol 125 μL

1x AB 875 μL