Alm:PCR
From OpenWetWare
Standard PCR reactions
- ~100 ng template
- ~100 pmole each primer
- buffer to 1X
- polymerase
- denature 94-95°C
- anneal 5°C less than lowest primer hyb temp
- extend 1’/kb to be amplified
5'-CATTAG can be useful to add to prevent exonuclease activity from degrading recognition sites