Agarose gel electrophoresis/Common issues

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There are a number of issues which plague gels far a variety of reasons. This page will contain some of the more common problems and show examples of gel images that have those problems.

So if you get a gel with something bizarre, keep the image and post it here so that others can learn from your mistake.

Possible unexpected bands:

  1. Supercoiled
  2. Nicked
    • Does anyone have good examples of these?
  3. Primer-dimers (how long?)
  4. Empty site in BB vector (~300bp band)