Difference between revisions of "Addition of 3' A overhangs to PCR products"
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Revision as of 20:41, 18 October 2008
If PCR is performed using a proofreading DNA polymerase, such as Pfu DNA polymerase, the product will have blunt ends. Taq DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning.
- Purified PCR product
- PCR buffer with Mg
- Taq DNA Polymerase
- Thermocycler or Heat block equilibrated at 72°C
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