AU Biomaterials Design Lab:Protocols/Starter Culture Media: Difference between revisions
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==Protocol== | ==Protocol== | ||
# Prepare LB in a 250mL Erlenmeyer flask | |||
## 0.875g of LB | |||
## 35mL of water | |||
# Cover the flask with foil | |||
# Autoclave | |||
# Allow the flask to cool | |||
# Add 35uL of 1000X Antibiotic | |||
# Inoculate with the bacteria you are culturing using one of the two following methods | |||
## Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask | |||
## Using sterile methods, add 5uL of a glycerol stock to the flask | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
==Notes== | ==Notes== | ||
==References== | ==References== | ||
Revision as of 11:45, 10 August 2011
Description
Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins.
Protocol
- Prepare LB in a 250mL Erlenmeyer flask
- 0.875g of LB
- 35mL of water
- Cover the flask with foil
- Autoclave
- Allow the flask to cool
- Add 35uL of 1000X Antibiotic
- Inoculate with the bacteria you are culturing using one of the two following methods
- Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask
- Using sterile methods, add 5uL of a glycerol stock to the flask
- Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm
