Difference between revisions of "AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol"

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(New page: ==Materials== * primers * plasmid *dexynucleoside triphosphate mixture (dNTPs) ==Equipment== PCR tubes temperature cycler ==Directions== *for DNA vectors (plasmids) of less than 10 kba...)
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Revision as of 08:56, 15 October 2012

Materials

  • primers
  • plasmid
  • dexynucleoside triphosphate mixture (dNTPs)

Equipment

PCR tubes temperature cycler

Directions

  • for DNA vectors (plasmids) of less than 10 kbases)
    • The primers must each be at 100-200 nh/mL
    • dNTPS 100-250 μM of each dNTPs
    • denaturing temperature is 95 oC
    • extension temperature is 72 oC