AU Biomaterials Design Lab:Protocols/Expression Culture Media: Difference between revisions
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(New page: ==Description== Starter cultures enable us to get a jump-start on culturing bacteria to log-phase while we are expressing proteins. We will usually make 4 starter cultures for our expressi...) |
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==Description== | ==Description== | ||
The expression culture will be able to support a large number of bacteria as they express our protein of interest. We will usually make 4 flasks-worth of expression culture. | |||
==Protocol== | ==Protocol== | ||
# Prepare [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] in a | *This needs to be done the day before protein expression | ||
## | # Prepare [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] in a 2.8L Fernbach flask | ||
## | ## 25g of LB | ||
## 1L of water | |||
# Cover the flask with foil | # Cover the flask with foil | ||
# Autoclave | # Autoclave (You can only fit two flasks into the autoclave at one time so you need to plan accordingly and perform two runs in the autoclave) | ||
# Allow the flask to cool | # Allow the flask to cool | ||
# Add | # Add 1mL of 1000X Antibiotic | ||
==Notes== | ==Notes== | ||
==References== | ==References== | ||
Latest revision as of 16:38, 11 August 2011
Description
The expression culture will be able to support a large number of bacteria as they express our protein of interest. We will usually make 4 flasks-worth of expression culture.
Protocol
- This needs to be done the day before protein expression
- Prepare LB in a 2.8L Fernbach flask
- 25g of LB
- 1L of water
- Cover the flask with foil
- Autoclave (You can only fit two flasks into the autoclave at one time so you need to plan accordingly and perform two runs in the autoclave)
- Allow the flask to cool
- Add 1mL of 1000X Antibiotic
