AU Biomaterials Design Lab:Equipment/UV-2550 Shimadzu

From OpenWetWare
Revision as of 18:31, 28 November 2012 by Keyun Wang (talk | contribs) (Purpose of use)
Jump to: navigation, search


  • Shimadzu® UV-2550
  • Shimadzu® UV-2550 is a ultra-low stray light double monochromator instrument. It provides UV-vis spectral analysis for samples by the embedded double blazed grating double monochromator. High energy optical system, wide dynamic range, small beam size, law stray light are some of its advantages over other double monochromators.

Purpose of use


Sample Range


  • Beeghly 207
  • American University
  • Washington, DC, 20016
    • access to the laboratory is restricted; see Dr. Miller, Dr. Hartings or Dr. Fox for access.



  • How does one use this instrument.
  • Include any relevant links to OWW protocols
  • Include any relevant links to the manufacturer's protocols
  • Note to users: you may note any troubleshooting or log your use on the talk page.


  • Are there any safety considerations for this instrument?


TCC-240A Thermoelectrically Temperature Controlled Cell Holder

  • Uses Peltier effect for controlling the temperatures of the sample and reference sample. No thermostatic bath or cooling water is required, so the operation is quite simple and easy.
    • Number of cells: One each on the sample and reference sides.
    • Temperature control range: 7 to 60°C
    • Temperature display accuracy (difference from the true value): ±0.5°C
    • Temperature control precision (variation of temperature): ±0.1°C

Multicell Sample Compartment

  • Holds up to six 10 mm square cells. No temperature control capability.
    • Number of cells: 6 on the sample side, and 1 on the reference side

UVProbe Software

  • Allows data processing with customized paramaters
    • Multitasking (possible to execute data processing while measurement is being executed)
    • Customizable measurement screen layout (wavelengths, data display font and font size, colors, displayed number of rows)
    • GLP/GMP compliant (security, history)
    • Real-time concentration display


Setting Wavelength Range

  • 190nm to 1100nm

Measurement Wavelength Range

  • 190nm to 900nm, but can measure up to 1100 with choice with detector

Wavelength accuracy

  • ±0.3nm

Wavelength repeatability

  • ±0.1nm

Wavelength Scanning Speed

  • Wavelength slew rate: about 3200nm/min
  • Wavelength scan rate: about 900nm-160nm/min
  • Monitor scan rate: about 2500nm/min

Wavelength setting

  • At 1nm units for scan start and sea end wavelengths, and 0.1nm units for over wavelengths

Lamp Interchange wavelength

  • Auto switching synchronized with wavelength, switching range selectable between 282nm-393nm (0.1nm units)

Spectral bandwidth

  • 6-step switching along 0.1/0.2/0.5/1/2/5nm


  • Optimum response speed automatically set depending on bandwidth, minimum 0.1sec


  • 0.1nm

Stray light

  • Less than 0.0003% (220nm, Nal 10g/L solution)
  • Less than 0.0003% (340nm, UV-39 filter)

Photometric system

  • Double-beam, direct-ratio system with dynode feedback

Photometric modes

  • Absorbance(Abs.), transmittance(%), reflectance(%), energy(E)

Photometric range

  • Absorbance: -4 to 5 Abs
  • Transmittance, reflectance: 0.0 to 999.9%

Photometric accuracy

  • ±0.002 Abs (0-0.5 Abs)
  • ±0.004 Abs (0.5-1.0 Abs)
  • ±0.3%T (0-100% T) filter
  • Tested with NIST 903D standard

Photometric repeatability

  • ±0.001 Abs (0-0.5 Abs)
  • ±0.002 Abs (0.5-1.0 Abs)
  • ±0.1%T

Baseline flatness

  • ±0.001 Abs (excluding noise, using 2nm slit, and slow wavelength scanning speed)

Baseline correction

  • Auto correction using PC (stored baseline is automatically loaded when power is switched on, re-correction is possible)


  • 0.0004Abs/h (after power is on for 2 hours)

Temperature and humidity requirements

  • 15-35°C, 45-80%(no condensation, less than 70% above 30°C)

Light source

  • 50W halogen lamp (2,000 hours life), deuterium lamp (socket type), light source auto position adjustment built in.


  • Grating/Grating type double monochromator
  • Pre-monochromator: double-blazed holographic grating
  • Main monochromator: high-performance blazed holographic grating in aberration-corrected Czerny-Turner mounting


  • R928 Red-Sensitive Photomultiplier

Sample compartment

  • Internal dimensions: 150W x 260D x 120H (mm)
  • Distance between light beams: 100nm
  • Maximum light path length of cell: 100nm

"Power requirements"

  • AC100, 120, 220, 240V, switch selectable
  • 50/60Hz; 250 VA


  • 570W x 660D x 275H (mm)


  • About 36kg


Useful links