AU Biomaterials Design Lab:Equipment/UV-2550 Shimadzu

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  • Shimadzu UV-2550

Purpose of use

  • How does this instrument work?
  • What can this instrument be used for?


  • Beeghly 207
  • American University
  • Washington, DC, 20016
    • access to the laboratory is restricted; see Dr. Miller, Dr. Hartings or Dr. Fox for access.



  • How does one use this instrument.
  • Include any relevant links to OWW protocols
  • Include any relevant links to the manufacturer's protocols
  • Note to users: you may note any troubleshooting or log your use on the talk page.


  • Are there any safety considerations for this instrument?


Setting Wavelength Range

  • 190nm to 1100nm

Measurement Wavelength Range

  • 190nm to 900nm, but can measure up to 1100 with choice with detector

Wavelength accuracy

  • ±0.3nm

Wavelength repeatability

  • ±0.1nm

Wavelength Scanning Speed

  • Wavelength slew rate: about 3200nm/min
  • Wavelength scan rate: about 900nm-160nm/min
  • Monitor scan rate: about 2500nm/min

Wavelength setting

  • At 1nm units for scan start and sea end wavelengths, and 0.1nm units for over wavelengths

Lamp Interchange wavelength

  • Auto switching synchronized with wavelength, switching range selectable between 282nm-393nm (0.1nm units)

Spectral bandwidth

  • 6-step switching along 0.1/0.2/0.5/1/2/5nm


  • Optimum response speed automatically set depending on bandwidth, minimum 0.1sec


  • 0.1nm

Stray light

  • Less than 0.0003% (220nm, Nal 10g/L solution)
  • Less than 0.0003% (340nm, UV-39 filter)

Photometric system

  • Double-beam, direct-ratio system with dynode feedback

Photometric modes

  • Absorbance(Abs.), transmittance(%), reflectance(%), energy(E)

Photometric range

  • Absorbance: -4 to 5 Abs
  • Transmittance, reflectance: 0.0 to 999.9%

Photometric accuracy

  • ±0.002 Abs (0-0.5 Abs)
  • ±0.004 Abs (0.5-1.0 Abs)
  • ±0.3%T (0-100% T) filter
  • Tested with NIST 903D standard

Photometric repeatability

  • ±0.001 Abs (0-0.5 Abs)
  • ±0.002 Abs (0.5-1.0 Abs)
  • ±0.1%T

Baseline flatness

  • ±0.001 Abs (excluding noise, using 2nm slit, and slow wavelength scanning speed)

Baseline correction

  • Auto correction using PC (stored baseline is automatically loaded when power is switched on, re-correction is possible)


  • 0.0004Abs/h (after power is on for 2 hours)

Temperature and humidity requirements

  • 15-35°C, 45-80%(no condensation, less than 70% above 30°C)

Light source

  • 50W halogen lamp (2,000 hours life), deuterium lamp (socket type), light source auto position adjustment built in.


  • Grating/Grating type double monochromator
  • Pre-monochromator: double-blazed holographic grating
  • Main monochromator: high-performance blazed holographic grating in aberration-corrected Czerny-Turner mounting


  • R928 Red-Sensitive Photomultiplier

Sample compartment

  • Internal dimensions: 150W x 260D x 120H (mm)
  • Distance between light beams: 100nm
  • Maximum light path length of cell: 100nm

"Power requirements"

  • AC100, 120, 220, 240V, switch selectable
  • 50/60Hz; 250 VA


  • 570W x 660D x 275H (mm)


  • About 36kg


Useful links