AU Biomaterials Design Lab:Equipment/FPLC

Description

AKTApurifier UPC

• AKTApurifier is an automated liquid chromatography system.
• This is the UPC model which is accompanied by the UPC-900 for combined measurement of UV absorption, pH, and conductivity.
• This features flow rates up to:

AKTApurifier 100 (P-901): 100 ml/min at pressures up to 10 MPa.

AKTApurifier 10 (P-903): 10 ml/min at pressures up to 25 MPa.

• This has a compact separation unit. UNICORN control system version 5.10 is the computer running system which includes:

(1) a fraction collector

(2) a monitor for pH and conductivity measurement

Purpose of Use

• This is used for rapid purification of proteins at low to medium pressures.
• The system works by passing the sample through different flowpaths with specific affinities for the constituents in the sample.

Location

• Beeghly 207

• American University, Washington, DC

• access to the laboratory is restricted; please contact Dr. Miller, Dr. Hartings or Dr. Fox for access.

Contact

For further details and use of the instrument please contact the following faculty:

• Dr. Matt Hartings
• Dr. Abigail Miller

Safety

• The instrument should be kept indoors and away from dusty atmosphere or close to spraying water.
• Air inlet should NOT be blocked on the front panels or the outlets on the rear of the instrument.
• Do not use for in vitro purposes.
• Modules and components contain high voltage circuits that can give a lethal electric shock.
• UPC-900 and UV-900 monitor uses high intensity UV light. Do not disassemble while the lamp is on.
• Appropriate tubing should be used. Tightening of tubes should be ensured.
• Do not place waste on top of the instrument.
• Wear appropriate laboratory attire when handling equipment.
• The system should be flushed with bacteriostatic solution.
• Pressure settings should match the tubing placed on the instrument.