Difference between revisions of "ATc"

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(Preparing working concentration)
 
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anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
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anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
  
 
==Preparing working concentration==
 
==Preparing working concentration==
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<pre>
 
<pre>
  
Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
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Anhydrotetracycline:
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Prepare a 1,000 times stock solution
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100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
  
 
</pre>
 
</pre>
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MW = 468.22 g/mole.  Thus 1X for the recipe above = 214 nM.
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==References==
 
==References==
  

Latest revision as of 18:37, 17 May 2012

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

Preparing working concentration

According to Nallamsetty and Waugh (2007):


Anhydrotetracycline:
Prepare a 1,000 times stock solution:  
100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.

MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.

References

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.

[1]