Difference between revisions of "ATc"

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(Preparing working concentration)
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Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
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Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving  
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in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
  
 
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==References==
 
==References==
  

Revision as of 22:30, 23 February 2008

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.

Preparing working concentration

According to Nallamsetty and Waugh (2007):


Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving 
in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.

References

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.

[1]