Difference between revisions of "840:153g:Projects/project27/2012/11/13"

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==Entry title==
 
==Entry title==
PCR products from 11/8 were checked on gel electrophoresis.  Again, no distinct bands were shown by the products amplified by either primer set.  The negative control, in which water was used instead of primers, also showed no amplification.  The positive control, in which a known template and primer set was used, showed a distinct band at ~500bp. The two controls indicate that the PCR procedure was adequate for amplification and that there was no significant contamination.  Lanes that included a 1:10 dilution of template DNA showed no amplification.  Lanes that included 5μL template DNA and lanes with products with annealing temperatures of 45°C, 47°C, and 50°C all showed smearing with no distinct bands visible.
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* PCR products from 11/8 were checked on gel electrophoresis.  Again, no distinct bands were shown by the products amplified by either primer set.  The negative control, in which water was used instead of primers, also showed no amplification.  The positive control, in which a known template and primer set was used, showed a distinct band at ~500bp. The two controls indicate that the PCR procedure was adequate for amplification and that there was no significant contamination.  Lanes that included a 1:10 dilution of template DNA showed no amplification.  Lanes that included 5μL template DNA and lanes with products with annealing temperatures of 45°C, 47°C, and 50°C all showed smearing with no distinct bands visible.
 
[[Image:MmoPCR1.jpg]]
 
[[Image:MmoPCR1.jpg]]
  

Revision as of 13:47, 27 November 2012

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Entry title

  • PCR products from 11/8 were checked on gel electrophoresis. Again, no distinct bands were shown by the products amplified by either primer set. The negative control, in which water was used instead of primers, also showed no amplification. The positive control, in which a known template and primer set was used, showed a distinct band at ~500bp. The two controls indicate that the PCR procedure was adequate for amplification and that there was no significant contamination. Lanes that included a 1:10 dilution of template DNA showed no amplification. Lanes that included 5μL template DNA and lanes with products with annealing temperatures of 45°C, 47°C, and 50°C all showed smearing with no distinct bands visible.

MmoPCR1.jpg