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Target Gene Amplification

  • Ran PCR reactions using M. trichosporium genomic DNA for template, 27F_mmoXY and 27R_mmoXY promoters to amplify XY gene segment, and 27F_mmoBZDC and 27R_mmoBZDC promoters to amplify BZDC gene segment. Each promoter pair was run at three different ligation temperatures, 55, 60, and 65. The purpose of these reactions is to amplify the two target gene regions, adding biobricks, for insertion into plasmid vectors. Next session, PCR products will be run on a gel to confirm lengths of amplified regions.