Difference between revisions of "840:153g:Projects/project27/2012/10/30"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Promoter Ligation)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Promoter Ligation==
 
==Promoter Ligation==
* Put together ligation reactions between the J23100 promoter and both the pSB1K3 and J62001 plasmid backbones.  Promoter solutions used were the working solution of 10um, 50x diluted working solution, and 500x diluted working solution.  Each was combined with an equal volume of plasmid solution in a ligation mix.  The purpose of this procedure was to add a promoter part, surrounded by biobricks, to each plasmid backbone so that target genes can be inserted behind the promoter.
+
* Put together ligation reactions between the J23100 promoter and both the pSB1K3 and J61002 plasmid backbones.  Promoter solutions used were the working solution of 10um, 50x diluted working solution, and 500x diluted working solution.  Each was combined with an equal volume of plasmid solution in a ligation mix.  The purpose of this procedure was to add a promoter part, surrounded by biobricks, to each plasmid backbone so that target genes can be inserted behind the promoter.
  
  

Revision as of 13:41, 5 December 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Promoter Ligation

  • Put together ligation reactions between the J23100 promoter and both the pSB1K3 and J61002 plasmid backbones. Promoter solutions used were the working solution of 10um, 50x diluted working solution, and 500x diluted working solution. Each was combined with an equal volume of plasmid solution in a ligation mix. The purpose of this procedure was to add a promoter part, surrounded by biobricks, to each plasmid backbone so that target genes can be inserted behind the promoter.