Difference between revisions of "840:153g:Projects/project27/2012/10/11"

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(RFP)
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==RFP==
 
==RFP==
* Discovered mRFP gene in plasmids; designed oligo-nucleotides in order to synthesize J23100 promoter.  This is to remove the promoter-RFP complex from each plasmid and insert our target promoter.
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* Discovered mRFP gene in plasmids.  Cultures containing pSB1K3 and those containing J61002 were red in color.  A 1022bp insert in pSB1K3 contains the parts R0010, B0034, E1010, and B0015.  A 845bp insert in J61002 contains J23100, E1010, and B0015.  We designed oligo-nucleotides, 27F_J23100 and 27R_J23100, in order to synthesize J23100 promoter.  We will digest out the promoter-RFP complex from the plasmid backbones and insert our target promoter
 
 
  
 
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Revision as of 19:02, 18 October 2012

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RFP

  • Discovered mRFP gene in plasmids. Cultures containing pSB1K3 and those containing J61002 were red in color. A 1022bp insert in pSB1K3 contains the parts R0010, B0034, E1010, and B0015. A 845bp insert in J61002 contains J23100, E1010, and B0015. We designed oligo-nucleotides, 27F_J23100 and 27R_J23100, in order to synthesize J23100 promoter. We will digest out the promoter-RFP complex from the plasmid backbones and insert our target promoter