Difference between revisions of "840:153g:Projects/project27/2012/10/09"

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(Digestion Enzyme Test)
(Digestion Enzyme Test)
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* Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min.  Results were checked on a gel, compared to undigested plasmid.
 
* Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min.  Results were checked on a gel, compared to undigested plasmid.
  
[[Image:Digest3.jpg]]
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[[Image:Digest3.jpg|400px]]
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Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively.  Lane 6 contained undigested plasmid.  Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively.  The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).
  
  

Revision as of 20:10, 18 October 2012

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Digestion Enzyme Test

  • Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min. Results were checked on a gel, compared to undigested plasmid.

Digest3.jpg

Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively. Lane 6 contained undigested plasmid. Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively. The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).