840:153g:Projects/project27/2012/10/09: Difference between revisions
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* Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min. Results were checked on a gel, compared to undigested plasmid. | * Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min. Results were checked on a gel, compared to undigested plasmid. | ||
[[Image:Digest3.jpg]] | [[Image:Digest3.jpg|400px]] | ||
Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively. Lane 6 contained undigested plasmid. Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively. The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6). | |||
Revision as of 20:10, 18 October 2012
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Digestion Enzyme Test
Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively. Lane 6 contained undigested plasmid. Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively. The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).
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