Difference between revisions of "840:153g:Projects/project27/2012/09/20"

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==Entry title==
 
==Entry title==
* Transformed chemically competent ''E. coli'' cells with pSB1A3 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control)
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* Transformed chemically competent ''E. coli'' cells with J62001 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control)
 
* After transformation, cultures were plated in the following quantities on antibiotic-containing media:
 
* After transformation, cultures were plated in the following quantities on antibiotic-containing media:
  
   - pSB1A3(& J23100): 200ul on ampicillin
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   - J62001(& J23100): 200ul on ampicillin
  
 
   - pSB1K3: 200ul on kanamycin
 
   - pSB1K3: 200ul on kanamycin

Revision as of 16:01, 5 December 2012

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Entry title

  • Transformed chemically competent E. coli cells with J62001 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control)
  • After transformation, cultures were plated in the following quantities on antibiotic-containing media:
 - J62001(& J23100): 200ul on ampicillin
 - pSB1K3: 200ul on kanamycin
 - pSB1A7: 50ul on ampicillin
           50ul on kanamycin
           50ul on no antibiotic
 - dH2O: 50ul on ampicillin
         50ul on kanamycin
         50ul on no antibiotic