Difference between revisions of "840:153g:Projects/project27/2012/09/20"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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* After transformation, cultures were plated in the following quantities on antibiotic-containing media:
 
* After transformation, cultures were plated in the following quantities on antibiotic-containing media:
  
   - pSB1A3(& J23100): 200ul on ampicillin
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   - J62001(& J23100): 200ul on ampicillin
  
 
   - pSB1K3: 200ul on kanamycin
 
   - pSB1K3: 200ul on kanamycin

Latest revision as of 21:02, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
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Entry title

  • Transformed chemically competent E. coli cells with J62001 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control)
  • After transformation, cultures were plated in the following quantities on antibiotic-containing media:
 - J62001(& J23100): 200ul on ampicillin
 - pSB1K3: 200ul on kanamycin
 - pSB1A7: 50ul on ampicillin
           50ul on kanamycin
           50ul on no antibiotic
 - dH2O: 50ul on ampicillin
         50ul on kanamycin
         50ul on no antibiotic