Difference between revisions of "840:153g:Projects/project26/2012/10/11"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==DNA Extraction, Reconsitute Primers==
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==DNA Extraction==
* On Tuesday we ran a gel on disgested and undigested snake DNA. There were no significant bands of undigested DNA. We are going to try and change a few things like reducing our dilution buffer and adding more tissue. We are also going to run a PCR on the sample anyway.  On Thursday we started another extraction along with changes to the protocol.  
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We started to run our DNA samples with PCR after seeing a precipitate in the DNA extraction tubes. We were hopeful we would have DNA. After running the snake DNA samples through the gel we found no evidence of DNA.
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1000 Bp ladder in the middle lane, with no visible DNA present in the other lanes.
  
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[[Image:!OCT9.tif]]
  
 
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Revision as of 08:46, 4 December 2012

Owwnotebook icon.png Cloning of Atrolysin A from Crotalus atrox <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA Extraction

We started to run our DNA samples with PCR after seeing a precipitate in the DNA extraction tubes. We were hopeful we would have DNA. After running the snake DNA samples through the gel we found no evidence of DNA. 1000 Bp ladder in the middle lane, with no visible DNA present in the other lanes.

File:!OCT9.tif