Difference between revisions of "840:153g:Projects/project26/2012/10/04"

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(DNA Extraction, Reconsitute Primers)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==DNA Extraction, Reconsitute Primers==
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==DNA Extraction, Reconstitute Primers==
* On Tuesday we ran a gel on disgested and undigested snake DNA.  There were no significant bands of undigested DNA. We are going to try and change a few things like reducing our dilution buffer and adding more tissue. We are also going to run a PCR on the sample anyway.  On Thursday we started another extraction along with changes to the protocol.  
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* On Tuesday we ran a gel on digested and undigested snake DNA.  There were no significant bands of either sample. We are going to try and change a few things like reducing our dilution buffer and adding more tissue. We are also going to run a PCR on the sample anyway.  On Thursday we started another extraction along with changes to the protocol.  
The lane on the far left is our Digested DNA sample along with the third one from the left and the other 2 lanes are Undigested DNA samples. Middle Lane is a 500 bp ladder.
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The lane on the far left is our Digested DNA sample along with the third one from the left and the other 2 lanes are Undigested DNA samples, and the middle lane is a 1000 Bp ladder.
 
[[Image:!GROUP26OCT2.tif]]
 
[[Image:!GROUP26OCT2.tif]]
 
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Revision as of 08:44, 4 December 2012

Owwnotebook icon.png Cloning of Atrolysin A from Crotalus atrox <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA Extraction, Reconstitute Primers

  • On Tuesday we ran a gel on digested and undigested snake DNA. There were no significant bands of either sample. We are going to try and change a few things like reducing our dilution buffer and adding more tissue. We are also going to run a PCR on the sample anyway. On Thursday we started another extraction along with changes to the protocol.

The lane on the far left is our Digested DNA sample along with the third one from the left and the other 2 lanes are Undigested DNA samples, and the middle lane is a 1000 Bp ladder. File:!GROUP26OCT2.tif