Difference between revisions of "840:153g:Projects/project26/2012/08/30"

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(Locating Shed)
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==Locating Shed==
 
==Locating Shed==
* After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone. Atrolysin A is the toxin. We were unable to find a dna sequence but found an RNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.
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* After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone, it is the atrolysin A toxin. We were unable to find a DNA sequence but found an mRNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.
  
  

Revision as of 09:36, 4 December 2012

Owwnotebook icon.png Cloning of Atrolysin A from Crotalus atrox <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Locating Shed

  • After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone, it is the atrolysin A toxin. We were unable to find a DNA sequence but found an mRNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.