840:153g:Projects/project25/2012/11/01: Difference between revisions
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This week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence. | This week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence. | ||
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Revision as of 14:40, 1 November 2012
 Cloning of mreB from Caulobacter crescentus into E. coli
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Exp. 5 Digestion of Mini Prep and Exp. 6 Preparatory Digest & PurificationThis week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence.
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