Difference between revisions of "840:153g:Projects/project25/2012/09/27"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of mreB gene from ''Caulobacter crescentus''</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==DNA Extraction/PCR==
  
  Bess Lippmann
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On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any.  We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1.  This is a picture of the gel: [[http://openwetware.org/wiki/Image:2012-09-27_16-23-27_929.jpg]].  The first well is the marker, well 2 is whole DNA, and well 3 is digested DNA.  This shows us that we have about the appropriate size of DNA  and it is good to work with.  We also made our stock solutions of our extended primers.
  Colby Swanson
 
  
Cloning mreB gene from Caulobacter Crescentus into E. Coli
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[[Image:2012-09-27_16-23-27_929.jpg|400px]]
  
    On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any.  We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1This is a picture of the gel: [[http://openwetware.org/wiki/Image:2012-09-27_16-23-27_929.jpg]]The first well is the marker, well 2 is whole DNA, and well 3 is digested DNAThis shows us that we have about the appropriate size of DNA  and it is good to work with.  We also made our stock solutions of our extended primers.
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On Thursday, 9/27, we planned out and executed our PCR reaction set.  We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. AxelWe ran 3 sets of our DNA samples with different annealing temperatures1 set with 65°C, 1 with 60°C, and 1 with 55°CThe reactions were to run overnight and stored at 4°C (in freezer) for the weekend.  We will run these out on a gel Tuesday, 10/2, to look at our results.
  
    On Thursday, 9/27, we planned out and executed our PCR reaction set.  We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. Axel.  We ran 3 sets of our DNA samples with different annealing temperatures.  1 set with 65°C, 1 with 60°C, and 1 with 55°C.  The reactions were to run overnight and stored at 4°C (in freezer) for the weekend.  We will run these out on a gel Tuesday, 10/2, to look at our results.
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[[category:OWWLabNotebookV1]]

Revision as of 11:55, 2 October 2012

Owwnotebook icon.png Cloning of mreB gene from Caulobacter crescentus <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA Extraction/PCR

On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any. We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1. This is a picture of the gel: [[1]]. The first well is the marker, well 2 is whole DNA, and well 3 is digested DNA. This shows us that we have about the appropriate size of DNA and it is good to work with. We also made our stock solutions of our extended primers.

2012-09-27 16-23-27 929.jpg

On Thursday, 9/27, we planned out and executed our PCR reaction set. We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. Axel. We ran 3 sets of our DNA samples with different annealing temperatures. 1 set with 65°C, 1 with 60°C, and 1 with 55°C. The reactions were to run overnight and stored at 4°C (in freezer) for the weekend. We will run these out on a gel Tuesday, 10/2, to look at our results.