Difference between revisions of "840:153g:Projects/project25/2012/09/27"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of mreB gene from ''Caulobacter crescentus''</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==DNA Extraction/PCR==
  
    Name of student
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On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any.  We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1.  This is a picture of the gel: [[http://openwetware.org/wiki/Image:2012-09-27_16-23-27_929.jpg]].  The first well is the marker, well 2 is whole DNA, and well 3 is digested DNA.  This shows us that we have about the appropriate size of DNA  and it is good to work with.  We also made our stock solutions of our extended primers.
    Name of student
 
  
Project Name and Description
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[[Image:2012-09-27_16-23-27_929.jpg|400px]]
  
    Explain your experimental design here
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On Thursday, 9/27, we planned out and executed our PCR reaction set.  We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. Axel.  We ran 3 sets of our DNA samples with different annealing temperatures.  1 set with 65°C, 1 with 60°C, and 1 with 55°C.  The reactions were to run overnight and stored at 4°C (in freezer) for the weekend. We will run these out on a gel Tuesday, 10/2, to look at our results.
    List all the steps that are needed to complete your project
 
    Do not go into experimental details and don't list procedures. Just list the major steps necessary to complete your project
 
    Please also make yourself familiar with uploading pictures and *.ppt files
 
  
Important Results and Milestones
 
  
    keep track of your most important results and refer to the corresponding page in your notebook
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    upload important pictures (don't forget to label them! Powerpoint is very convenient). Remember: these will become quite handy later in your summary report or final presentation. If you do label and upload the pictures as soon as you got them, your summary report can be written much more effortlessly (do you usually procrastinate? This is chance to do some work before hand that frees you up for finals week).  
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Recent changes
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[[category:OWWLabNotebookV1]]

Revision as of 12:55, 2 October 2012

Owwnotebook icon.png Cloning of mreB gene from Caulobacter crescentus <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA Extraction/PCR

On Tuesday, 9/25, we finished our DNA Extraction protocol and ran out the 'DNA' with gel Electrophoresis to see if we actually had any. We ran a 100 bp plus Marker, our genome DNA, and our DNA digested with ECOR1. This is a picture of the gel: [[1]]. The first well is the marker, well 2 is whole DNA, and well 3 is digested DNA. This shows us that we have about the appropriate size of DNA and it is good to work with. We also made our stock solutions of our extended primers.

2012-09-27 16-23-27 929.jpg

On Thursday, 9/27, we planned out and executed our PCR reaction set. We ran out a negative control, primers without DNA template, and a positive control, plasmid 1A7 with VF2/VR primers which were provided by our instructor Dr. Axel. We ran 3 sets of our DNA samples with different annealing temperatures. 1 set with 65°C, 1 with 60°C, and 1 with 55°C. The reactions were to run overnight and stored at 4°C (in freezer) for the weekend. We will run these out on a gel Tuesday, 10/2, to look at our results.