840:153g:Projects/project24/2012/10/25

From OpenWetWare
Revision as of 16:26, 25 October 2012 by Sanju Timilsina (talk | contribs) (Title here)
Jump to: navigation, search
Owwnotebook icon.png Project Name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Agarose gel electrophorosis for extraced DNA sample

On Tuesday, we completed DNA extraction process. We also ran 0.8% Agarose gel to quantify the DNA that we extracted. We ran 12 samples ( 6 undigested and 6 EcoRI digested) on the gel. Due to some technical problem we couldn't see the bands under UV the same day. So, we rapped our gel into a air tight plastic bag and stored it at 4 degree C. On Wednesday, morning we saw the gel under UV. Out of 12 6 samples gave good bands.DNA 3 undigested didn't showed any bands however, its digested sample showed good bands. So, we decided to run Sample 3 into the gel one more time in our next lab. Also, we decided to mix sample DNA 1 and DNA 5 because they both gave similar intensities.

On Thursday,we had to attend a workshop so we didn't do any lab.